Default common primers offered by Wyzer Biosciences*:
  • M13F = TGT AAA ACG ACG GCC AGT (116060)
  • M13R = GGA AAC AGC TAT GAC CAT GA (116108)
  • SP6 = ATT TAG GTG ACA CTA TAG AAT (116112)
  • T7 Promoter = TGT AAT ACG ACT CAC TAT AGG G (116120)
  • T7 Terminator = GCT AGT TAT TGC TCA GCG G (128861)
  • T3 Promoter = GAA ATT AAC CCT CAC TAA AGG G (116117)

Common primers listed in IDT's web site:
  • M13F = GTA AAA CGA CGG CCA GT
  • M13F = CGC CAG GGT TTT CCC AGT CAC GAC
  • M13 R = CAG GAA ACA GCT ATG AC
  • M13 R = AGC GGA TAA CAA TTT CAC ACA GG
  • SP6 Promoter = TAC GAT TTA GGT GAC ACT ATA G
  • SP6 Upstream = ATT TAG GTG ACA CTA TAG
  • T7 Promoter = TAA TAC GAC TCA CTA TAG GG
  • T7 terminator = GCT AGT TAT TGC TCA GCG G
  • T3 Promoter = AAT TAA CCC TCA CTA AAG GG

Common primers listed in Invitrogen's web site:
  • M13F = GTA AAA CGA CGG CCA G
  • M13R = CAG GAA ACA GCT ATG AC
  • SP6 = GAT TTA GGT GAC ACT ATA G
  • T7 = TAA TAC GAC TCA CTA TAG GG
  • T3 Promoter = ATT AAC CCT CAC TAA AGG GA
Common primers listed in Promega's web site:
  • M13F = GTT TTC CCA GTC ACG AC
  • M13F = CGC CAG GGT TTT CCC AGT CAC GAC
  • M13R = CAG GAA ACA GCT ATG AC
  • M13R = TCA CAC AGG AAA CAG CTA TGA C
  • SP6 = TAT TTA GGT GAC ACT ATA G
  • T7 Promoter = TAA TAC GAC TCA CTA TAG GG


For additional list of other common primers, please click here

*Note: When applicable, we will use our common primers unless specified otherwise. A customer can request to add a specific primer to our list of "common" primers. We expect that such a primer will be used repeatedly over time.