Sequencing of difficult templates:
Occasionally, a submitted DNA template will not yield good quality data when using standard sequencing protocols and from the sequencing point of view we refer to such a template as "difficult." The most common sequence combinations that render a template or a segment therein as difficult to sequence are:
  • over 70% GC-rich regions
  • hairpins
  • long GC homopolymers
  • long poly A/T tails
  • di-and tri-nucleotide repeat stretches
  • long stretches of di-nucleotides that are not repeats.
  • long direct repeats

We have developed a number of proprietary protocols to effectively deal with each category of difficult templates. We ask that if you are aware of such conditions in your templates that you inform us. It will speed up data turnaround and reduce your sequencing costs.